Journal: Frontiers in Immunology

Article Title: Rab7 inhibitor enhances stem cell differentiation into keratinocyte-like cells with anti-inflammatory properties

doi: 10.3389/fimmu.2025.1503007

Figure Lengend Snippet: Effect of Rab7 inhibition on the viability and morphology of ASCs. (A) Viability of ASCs after treatment with different concentrations of CID-1067700 at 24 and 72 hours. No significant changes in cell viability were observed. (B) Representative bright-field images illustrating the morphological changes in ASCs after 10 days of treatment with differentiation media (DM) with and without 40 μM CID-1067700, in comparison to undifferentiated ASCs. Scale bars = 100 μm.

Article Snippet: The adipose-derived mesenchymal stem cell (ASCs) line, ASC52telo (SCRC-4000, ATCC) immortalized with human telomerase reverse transcriptase (hTERT), were selected for their consistent stem cell properties and reproducibility.

Techniques: Inhibition, Comparison

Journal: Frontiers in Immunology

Article Title: Rab7 inhibitor enhances stem cell differentiation into keratinocyte-like cells with anti-inflammatory properties

doi: 10.3389/fimmu.2025.1503007

Figure Lengend Snippet: Rab7 inhibition leads to transcriptomic changes in ASCs. (A) Principal component analysis (PCA) of the transcriptomic profiles of cells treated as control (DM), with vehicle (DMSO), or with CID. PCA revealed clustering of the vehicle group with DM group, indicating a high degree of similarity between these two groups. (B) Volcano plot showed the differentially expressed genes (DEGs) in the CID-treated ASCs compared to those treated with DM. Downregulated genes are shown in blue, upregulated genes are in red, while insignificantly regulated genes are in grey.

Article Snippet: The adipose-derived mesenchymal stem cell (ASCs) line, ASC52telo (SCRC-4000, ATCC) immortalized with human telomerase reverse transcriptase (hTERT), were selected for their consistent stem cell properties and reproducibility.

Techniques: Inhibition, Control

Journal: Frontiers in Immunology

Article Title: Rab7 inhibitor enhances stem cell differentiation into keratinocyte-like cells with anti-inflammatory properties

doi: 10.3389/fimmu.2025.1503007

Figure Lengend Snippet: Impact of Rab7 inhibition on signalling pathways, biological processes, and upstream regulators in ASCs. (A) Chord Diagram of the top six significantly enriched pathways. (B) The top five enriched GO biological processes among the DEGs in the CID-treated ASCs. (C) Predicted activated or (D) inhibited upstream regulators for the DEGs in CID-treated ASCs compared to the control group. These predictions were made using the iPathwayGuide software.

Article Snippet: The adipose-derived mesenchymal stem cell (ASCs) line, ASC52telo (SCRC-4000, ATCC) immortalized with human telomerase reverse transcriptase (hTERT), were selected for their consistent stem cell properties and reproducibility.

Techniques: Inhibition, Control, Software

Journal: Frontiers in Immunology

Article Title: Rab7 inhibitor enhances stem cell differentiation into keratinocyte-like cells with anti-inflammatory properties

doi: 10.3389/fimmu.2025.1503007

Figure Lengend Snippet: Validation of the expression levels of the differentially expressed genes identified by microarray with qPCR. (A) Bar chart displaying the relative expression levels of seven selected DEGs ( COL81A, COL1A1, ITGB8, MGP, FGF7, VCAM1, and HMOX-1) . Bars represent the log2-fold changes in gene expression in CID-1067700-treated ASCs (CID) compared to the control group (DM). (B) Validation of the expression levels of the selected genes by qPCR. The expression levels are normalized to GAPDH and calculated relative to the expression of ASCs treated with DM only using the 2 -∆∆Cq method. Data are presented as mean ± standard deviation (SD) of three experimental replicates. The vehicle control was added to the qPCR to validate similarity with the negative control.

Article Snippet: The adipose-derived mesenchymal stem cell (ASCs) line, ASC52telo (SCRC-4000, ATCC) immortalized with human telomerase reverse transcriptase (hTERT), were selected for their consistent stem cell properties and reproducibility.

Techniques: Biomarker Discovery, Expressing, Microarray, Gene Expression, Control, Standard Deviation, Negative Control

Journal: Frontiers in Immunology

Article Title: Rab7 inhibitor enhances stem cell differentiation into keratinocyte-like cells with anti-inflammatory properties

doi: 10.3389/fimmu.2025.1503007

Figure Lengend Snippet: Rab7 Inhibition induces changes in the proteomic profile of ASCs. The upper left image shows the original scan of the protein array membrane. Noticeable reduction in the levels of inflammatory cytokines IL-1α, IL-8, IL-17A, and IL-32 were observed in CID-treated cells, whereas the expression level of EGF was increased. Bars represent the mean fold change in protein expression ± standard deviation (SD) of two experimental replicates in response to CID treatment compared to the control (DM).

Article Snippet: The adipose-derived mesenchymal stem cell (ASCs) line, ASC52telo (SCRC-4000, ATCC) immortalized with human telomerase reverse transcriptase (hTERT), were selected for their consistent stem cell properties and reproducibility.

Techniques: Inhibition, Protein Array, Membrane, Expressing, Standard Deviation, Control

Journal: Frontiers in Immunology

Article Title: Rab7 inhibitor enhances stem cell differentiation into keratinocyte-like cells with anti-inflammatory properties

doi: 10.3389/fimmu.2025.1503007

Figure Lengend Snippet: Rab7 Inhibition enhances the expression of epithelial differentiation markers in ASCs. The latter were cultured with differentiation media for ten days with and without CID-1067700, and immunocytochemistry analysis was performed to assess the expression of Cytokeratin 5 and 14, as well as Filaggrin and P63. Nuclei were counterstained with DAPI (blue). The bars indicate the mean fluorescence intensity unit (FU) per cell ± SD of 3 replicates. Asterisks represent significant difference compared to cells treated with differentiation media (DM) only (* p<0.5, ** p<0.01). Scale bars = 100 μm.

Article Snippet: The adipose-derived mesenchymal stem cell (ASCs) line, ASC52telo (SCRC-4000, ATCC) immortalized with human telomerase reverse transcriptase (hTERT), were selected for their consistent stem cell properties and reproducibility.

Techniques: Inhibition, Expressing, Cell Culture, Immunocytochemistry, Fluorescence

Journal: Frontiers in Immunology

Article Title: Rab7 inhibitor enhances stem cell differentiation into keratinocyte-like cells with anti-inflammatory properties

doi: 10.3389/fimmu.2025.1503007

Figure Lengend Snippet: Effect of Rab7 inhibition on the expression of keratinocyte markers in ASCs cells. The gene expression of (A) Vimentin, (B) Filaggrin, (C) Cytokeratin 5, and (D) Cytokeratin 14 was measured by Real-time PCR. The expression levels were normalized to GAPDH expression and calculated relative to cells treated with differentiation media (DM) alone. Asterisks indicate a significant difference compared to cells treated with DM (* p<0.05, ** p<0.01, *** p<0.001).

Article Snippet: The adipose-derived mesenchymal stem cell (ASCs) line, ASC52telo (SCRC-4000, ATCC) immortalized with human telomerase reverse transcriptase (hTERT), were selected for their consistent stem cell properties and reproducibility.

Techniques: Inhibition, Expressing, Gene Expression, Real-time Polymerase Chain Reaction

Journal: Journal of Personalized Medicine

Article Title: The Effect of Ionizing Irradiation on the Autotaxin-Lysophasphatidic Acid Axis and Interleukin-6/8 Secretion in Different Breast Cancer Cell Lines

doi: 10.3390/jpm14090968

Figure Lengend Snippet: Alterations in ATX expression on the mRNA level of ASC/TERT1 cells 24 h after stimulation with IL-6 ( A ) and IL-8 ( B ). The columns show the mean relative ATX expression (y-axis) in varying concentrations of IL-6 and IL-8 (x-axis) compared to YWHAZ and to the control group. Values were calculated using the 2 −ΔΔCT method, where 2 −ΔΔCT of control = 1. * p < 0.05 (Mann–Whitney U test).

Article Snippet: An ASC/TERT1 (human-adipose-tissue-derived telomerase-immortalized mesenchymal stem cell line) was purchased from Evercyte (Evercyte GmbH, Vienna, Austria) and cultivated in Endothelial Cell Growth Medium (EGM)-2 BulletKit (Lonza Group AG, Basel, Switzerland).

Techniques: Expressing, Control, MANN-WHITNEY